BLM anticorps (pSer144)

N° du produit ABIN5596801

N° du produit (Fournisseur): 600-401-j57

Cet anticorps anti-BLM est un anticorps Lapin Polyclonal détectant BLM dans WB, ELISA et IHC. Adapté pour Humain.

Aperçu rapide pour BLM anticorps (pSer144) (ABIN5596801)

Antigène: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BLM est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-BLM anticorps

Épitope: pSer144

N° du produit (Fournisseur): 600-401-j57

Fournisseur: Rockland

Fonction: BLM phospho S144 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with human and mouse based on 100 % sequence homology.

Attributs du produit: Synonyms: rabbit anti-BLM pS144 Antibody, DNA helicase, RecQ-like type 2, RECQ2, RECQL3, Bloom syndrome protein

Purification: Anti-BLM pS144 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogène:

Immunogen: Anti-BLM pS144 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Bloom syndrome protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-BLM pS144 antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~159 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur BLM

Antigène: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Autre désignation: BLM

Sujet: Background: BLM pS144 product is a member of the RecQ helicase protein family. The protein contains 1417 amino acids, including one ATP binding site, one DEAH box, and two putative nuclear localization signals, as well as phosphorylation sites. BLS protein is known to associate and be phosphorylated by the ATR (ataxia telangiectasia, rad3+) protein, in response to genetic stress. The Bloom's syndrome gene product is thought to play a role in the etiology of two major human health problems, each of which is a very common complication of Bloom's syndrome, namely cancer and diabetes. Anti-BLM pS144 antibody is ideal for researchers interested in Epigenetics and Nuclear Signaling or DNA Damage & Repair research.

UniProt: P54132

Pathways: Réparation de l'ADN