COL2A1 anticorps

N° du produit ABIN5596824

N° du produit (Fournisseur): 600-401-104-0.1

L’anticorps Lapin Polyclonal anti-COL2A1 a été validé pour WB, IHC, ELISA, IP, FM et DB et a été validé indépendamment. Il convient pour détecter COL2A1 dans des échantillons de Humain et Boeuf (Vache).

Aperçu rapide pour COL2A1 anticorps (ABIN5596824)

Antigène: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reactivité: Humain, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp COL2A1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM), Dot Blot (DB)

Détail du produit anti-COL2A1 anticorps

N° du produit (Fournisseur): 600-401-104-0.1

Fournisseur: Rockland

Fonction: Collagen Type II Antibody

Réactivité croisée (Details): Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Attributs du produit: Synonyms: rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody

Purification: Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Collagen Type II from human knee cartilage and bovine nasal cartilage.

Immunogen Type: Native Protein

Isotype: IgG

Informations sur le produit:

À quoi peut servir l'anticorps COL2 ABIN5596824 ? Cet anticorps polyclonal non conjugué de lapin COL2 convient à la détection du collagène de type 2 (COL2) par ELISA, immunohistochimie, immuniprécipitation et Western blotting. L'anticorps reconnaît COL2 chez l'homme, la souris, le rat, le bovin, le mouton et d'autres mammifères.

Quelles sont les données de validation disponibles pour cet anticorps COL2 ? Le produit a été validé indépendamment par NanoTemper Technologies en déterminant le profil de dépliage. Il y a actuellement 2 images de produit disponibles montrant les performances de l'anticorps COL2. Utilisez notre anticorps pour détecter COL2 de manière fiable.

Quelle est la fonction de COL2 ? Le collagène de type 2 (COL2) est spécifique des tissus cartilagineux. Il est essentiel pour le développement embryonnaire normal du squelette, pour la croissance linéaire et pour la capacité du cartilage à résister aux forces de compression. L'anticorps COL2 a pour fonction de détecter le collagène de type 2.

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50 - 1:400

Application Note: Anti-Collagen Type II has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Independent Validation (UP)

Validation #104083 (Unfolding Profile)

by: NanoTemper Technologies

No.: #104083

Date: 23.07.2019

Antigène: COL2

Numéro du lot: 41639

Application validée: Unfolding Profile

Contrôle positif: ABIN5596824

Conclusion:

Passed. ABIN5596824 showed T_i at 78.9°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

Validation Images

Unfolding profile of ABIN5596824. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 78.9°C.

Protocole

Full Protocol:

• Dilute ABIN5596824 in PBS buffer (Roth, 1058.1, Lot 285231988) to get a final volume of 30µl at a concentration of 0.1µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Date de péremption: 12 months

Détails sur COL2A1

Antigène: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Autre désignation: COL2A1

Sujet: Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

ID gène: 1280

NCBI Accession: NP_001835

UniProt: P02458

Pathways: Sensory Perception of Sound, Growth Factor Binding