COL2A1 anticorps (C-Term)

N° du produit ABIN5518709

Détail du produit anti-COL2A1 anticorps

Antigène: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Épitope: AA 1222-1241, C-Term

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp COL2A1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Fonction: Rabbit IgG polyclonal antibody for Collagen alpha-1(II) chain(COL2A1) detection. Tested with WB, IHC-F in Mouse,Rat.

Séquence: AFAGLGQREK GPDPMQYMRA

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Collagen alpha-1(II) chain(COL2A1) detection. Tested with WB, IHC-F in Mouse,Rat.
Gene Name: collagen, type II, alpha 1
Protein Name: Collagen alpha-1(II) chain

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen II1222-1241aa AFAGLGQREKGPDPMQYMRA), different from the related rat sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse, The detection limit for COL2A1 is approximately 1 ng/lane under reducing conditions.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(F).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-COL2A1 (ABIN5518709)

Jia, Ma, Lv, Ma, Xu, Yang, Tian, Wang, Sun, Xu, Fu, Zhao: "Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/β-catenin pathway activity." dans: Scientific reports, Vol. 6, pp. 27521, (2018) (PubMed).

Li, Zou, Wang, Wang, Li, Chen, Sun, Sun: "TGF-β1/Smad3 Signaling Pathway Mediates T-2 Toxin-Induced Decrease of Type II Collagen in Cultured Rat Chondrocytes." dans: Toxins, Vol. 9, Issue 11, (2018) (PubMed).

Liang, Sun, Wei, Zhang, Li, Xu, Shang, Deng, Zhao, Wei: "Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture." dans: BMC musculoskeletal disorders, Vol. 18, Issue 1, pp. 464, (2018) (PubMed).

Qin, Jin, Jiang, Li, Tan, Wu, Zheng, Zhao: "A Human Chondrocyte-Derived In Vitro Model of Alcohol-Induced and Steroid-Induced Femoral Head Necrosis." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 539-547, (2018) (PubMed).

Jiang, Zhong, Zheng, Zhao: "Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro." dans: International journal of molecular medicine, Vol. 41, Issue 4, pp. 2150-2158, (2018) (PubMed).

Zeng, Xiao, Deng, Li: "Chondroprotective Effects and Multitarget Mechanisms of Fu Yuan Capsule in a Rat Osteoarthritis Model." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 8985623, (2017) (PubMed).

Huang, Wu, Wang, Zheng, Zhao: "Protective effects of baicalin on rabbit articular chondrocytes in vitro." dans: Experimental and therapeutic medicine, Vol. 13, Issue 4, pp. 1267-1274, (2017) (PubMed).

Hou, Huang, Luo, Wang, Liu, Deng, Zhang, Liu, Chen: "MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR." dans: American journal of translational research, Vol. 9, Issue 11, pp. 4963-4973, (2017) (PubMed).

Tian, Zhong, Zheng, Zhang, Liu, Zhang, Liu: "Neuroleukin/Autocrine Motility Factor Receptor Pathway Promotes Proliferation of Articular Chondrocytes through Activation of AKT and Smad2/3." dans: Scientific reports, Vol. 5, pp. 15101, (2016) (PubMed).

Liu, Tian, Zhou, Wang, Gou, Zhang, Wang, Shen, Zhang, Zhang: "Protective effect of calcitonin on lumbar fusion-induced adjacent-segment disc degeneration in ovariectomized rat." dans: BMC musculoskeletal disorders, Vol. 16, pp. 342, (2016) (PubMed).

Tian, Zhong, Zhang, Yin, Zhang, Liu: "Microfluidics‑based optimization of neuroleukin‑mediated regulation of articular chondrocyte proliferation." dans: Molecular medicine reports, Vol. 13, Issue 1, pp. 67-74, (2016) (PubMed).

Gu, Li, Li, Yu, Wang, Ding, Liu: "Oral Resveratrol Prevents Osteoarthritis Progression in C57BL/6J Mice Fed a High-Fat Diet." dans: Nutrients, Vol. 8, Issue 4, pp. 233, (2016) (PubMed).

Li, Sun, Liu, Chen, Rong, Lou, Yang, Yang, Liu: "Use of a biological reactor and platelet-rich plasma for the construction of tissue-engineered bone to repair articular cartilage defects." dans: Experimental and therapeutic medicine, Vol. 12, Issue 2, pp. 711-719, (2016) (PubMed).

Liu, Lu, Wu, Zheng: "Chondroprotective effects of taurine in primary cultures of human articular chondrocytes." dans: The Tohoku journal of experimental medicine, Vol. 235, Issue 3, pp. 201-13, (2015) (PubMed).

Ye, Wu, Li, Li, Zheng, Liu, Ye: "Millimeter Wave Treatment Inhibits Apoptosis of Chondrocytes via Regulation Dynamic Equilibrium of Intracellular Free Ca (2+)." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 464161, (2015) (PubMed).

Cai, Zhang, Zhang, Ge, Dai, Tan, Roodrajeetsing, Cai: "Promoting Effects on Proliferation and Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Four "Kidney-Tonifying" Traditional Chinese Herbs." dans: BioMed research international, Vol. 2015, pp. 792161, (2015) (PubMed).

Sun, Meng, Li, Liu, Chen: "Construction of tissue-engineered laryngeal cartilage with a hollow, semi-flared shape using poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) as a scaffold." dans: Experimental and therapeutic medicine, Vol. 9, Issue 4, pp. 1482-1488, (2015) (PubMed).

Wang, Yao, Huang, Li, Xu: "Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage." dans: Experimental and therapeutic medicine, Vol. 10, Issue 3, pp. 995-1002, (2015) (PubMed).

Ouyang, Wang, Hong, Xin, Wang, Yang, Wang, Wang, Wei, Wang, Cui: "Establishment of a rat model for osteoarthritis resulting from anterior cruciate ligament rupture and its significance." dans: Experimental and therapeutic medicine, Vol. 10, Issue 6, pp. 2035-2038, (2015) (PubMed).

Liu, Liu, Lin, Wei, Zheng: "Effect of JEZTC, a synthetic compound, on proliferation and phenotype maintenance of rabbit articular chondrocytes in vitro." dans: In vitro cellular & developmental biology. Animal, Vol. 50, Issue 10, pp. 982-91, (2014) (PubMed).

Détails sur COL2A1

Antigène: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Autre désignation: COL2A1 (COL2A1 Produits)

Synonymes: anticorps ANFH, anticorps AOM, anticorps COL11A3, anticorps SEDC, anticorps Col2, anticorps Col2a, anticorps Col2a-1, anticorps Del1, anticorps Dmm, anticorps Lpk, anticorps M100856, anticorps Rgsc856, anticorps CG2A1A, anticorps COLLII, anticorps col2a1a, anticorps CB11, anticorps col2a1, anticorps coll2a1, anticorps fb38c06, anticorps fc10c01, anticorps wu:fb38c06, anticorps wu:fc10c01, anticorps collagen type II alpha 1 chain, anticorps collagen, type II, alpha 1, anticorps collagen, type II, alpha 1 L homeolog, anticorps collagen, type II, alpha 1 S homeolog, anticorps collagen, type II, alpha 1a, anticorps COL2A1, anticorps Col2a1, anticorps col2a1, anticorps col2a1.L, anticorps col2a1.S, anticorps col2a1a

Sujet: Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Synonyms: Alpha 1 type II collagen antibody|Alpha-1 type II collagen antibody|AOM antibody|Cartilage collagen antibody|Chondrocalcin antibody|CO2A1_HUMAN antibody|COL11A3 antibody|COL2A1 antibody|COL2A1 protein antibody|Collagen alpha 1(II) chain precursor antibody|Collagen II alpha 1 polypeptide antibody|Collagen type II alpha 1(primary osteoarthritis spondyloepiphyseal dysplasia congenital) antibody|MGC131516 antibody|SEDC antibody

UniProt: P28481

Pathways: Sensory Perception of Sound, Growth Factor Binding