GPD1 anticorps (HRP)

N° du produit ABIN5596858

Détail du produit anti-GPD1 anticorps (HRP)

Antigène: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GPD1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Glycerol-3-Phosphate-Dehydrogenase from other sources is unknown.

Purification: Glycerol-3-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle].

Immunogène:

Immunogen: Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Glycerol-3-Phosphate Dehydrogenase has been assayed against 1.0 μg of Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur GPD1

Antigène: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Autre désignation: Glycerol-3-Phosphate Dehydrogenase (GPD1 Produits)

Synonymes: anticorps GPDH, anticorps Gpd3, anticorps GPD-C, anticorps GPDH-C, anticorps HTGTI, anticorps CG9042, anticorps DROGPDHA, anticorps DmG3PDH, anticorps Dmel\\CG9042, anticorps G3PDH, anticorps G3pdh, anticorps GAPDH, anticorps GPD, anticorps GPDA, anticorps GPDH-1, anticorps Gdh, anticorps Gpd, anticorps alpha-GPD, anticorps alpha-GPDH, anticorps alpha-GPDH-1, anticorps alpha-Gpdh, anticorps alphaGPDH, anticorps alphaGpd, anticorps alphaGpdh, anticorps alphaGpdh-1, anticorps gpdh, anticorps gpdh-1, anticorps sn-Gpdh, anticorps AI747587, anticorps Gdc-1, anticorps Gdc1, anticorps mKIAA4010, anticorps gpd1, anticorps wu:fc30a07, anticorps zgc:63859, anticorps MGC79676, anticorps GPD1, anticorps LG3P, anticorps zgc:112197, anticorps Gpd1, anticorps gpd1h, anticorps gpd1l, anticorps wu:fc58b05, anticorps zgc:66051, anticorps zgc:85742, anticorps glycerol-3-phosphate dehydrogenase 1, anticorps Glycerol-3-phosphate dehydrogenase [NAD(+)], anticorps glycerol-3-phosphate dehydrogenase, anticorps Glycerol 3 phosphate dehydrogenase, anticorps glycerol-3-phosphate dehydrogenase 1 (soluble), anticorps glycerol-3-phosphate dehydrogenase 1 L homeolog, anticorps glycerol-3-phosphate dehydrogenase 1c, anticorps glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, anticorps glycerol-3-phosphate dehydrogenase 1a, anticorps glycerol-3-phosphate dehydrogenase 1b, anticorps Gpd1, anticorps gpdh-1, anticorps GPD1, anticorps Tb11.02.5280, anticorps Gpdh, anticorps gpd1.L, anticorps gpd1c, anticorps gpd1, anticorps LOC101071719, anticorps gpd1a, anticorps gpd1b

Sujet:

Synonyms: EC 1.1.1.8 antibody, FLJ26652 antibody, G3PD antibody, Gdc-1 antibody, Glycerphosphate dehydrogenase antibody, GPD-C antibody, Gpd1 protein antibody

Background: Glycerol-3-phosphate dehydrogenase serves as a major link between carbohydrate metabolism and lipid metabolism. Through the reduction of dihydroxyacetone phosphate into glycerol 3-phosphate, GPDH allows the prompt dephosphorylation of glycerol 3-phosphate into glycerol. It is also a major contributor of electrons to the electron transport chain in the mitochondria. GPDH is responsible for maintaining the redox potential across the inner mitochondrial membrane in glycolysis. Since glycerol is a main subunit in lipid metabolism, its abundance can easily lead to an increase in triglyceride accumulation at a cellular level. As a result, there is a tendency to form adipose tissue leading to an accumulation of fat that favors obesity. GPDH has also been found to play a role in Brugada syndrome. Mutations in the gene encoding GPD1 have been proven to cause defects in the electron transport chain. This conflict with NAD+/NADH levels in the cell is believed to contribute to defects in cardiac sodium ion channel regulation and can lead to a lethal arrythmia during infancy.

Gene Name: GPD1

ID gène: 100339469

UniProt: P08507