GAPDH anticorps

N° du produit ABIN559691

L’anticorps Souris Monoclonal anti-GAPDH a été validé pour WB, ELISA, DB et ISt. Il convient pour détecter GAPDH dans des échantillons de Humain, Souris, Rat, Lapin, Poulet, Hamster, Saccharomyces cerevisiae, Insectes et Bactéries. Il y a 3+ publications disponibles.

Aperçu rapide pour GAPDH anticorps (ABIN559691)

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Humain, Souris, Rat, Lapin, Poulet, Hamster, Saccharomyces cerevisiae, Insectes, Bactéries

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Immunostaining (ISt)

Clone: GA1R

Détail du produit anti-GAPDH anticorps

Specificité: Recognizes native and denatured forms of GAPDH (∼37kDa). GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster / GAPDH from other species may also be detectable

Purification: Protein A affinity chromatography from mouse ascites fluid

Immunogène: Recombinant GAPDH

Isotype: IgG1

Information d'application

Indications d'application: WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test

Restrictions: For Research Use only

Stockage

Concentration: 1mg/mL

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-GAPDH (ABIN559691)

Steinbrecht, Pfeifer, Herzog, Katzenberger, Schulz, Kammerer, Küpper: "HepG2-1A2 C2 and C7: Lentivirus vector-mediated stable and functional overexpression of cytochrome P450 1A2 in human hepatoblastoma cells." dans: Toxicology letters, Vol. 319, pp. 155-159, (2019) (PubMed).

Martínez-Moya, Ortega-González, González, Anzola, Ocón, Hernández-Chirlaque, López-Posadas, Suárez, Zarzuelo, Martínez-Augustin, Sánchez de Medina: "Exogenous alkaline phosphatase treatment complements endogenous enzyme protection in colonic inflammation and reduces bacterial translocation in rats." dans: Pharmacological research : the official journal of the Italian Pharmacological Society, Vol. 66, Issue 2, pp. 144-53, (2012) (PubMed).

Gandhi, Takahashi, Virtanen, Syed, Davey, Mahomed: "Microarray analysis of the infrapatellar fat pad in knee osteoarthritis: relationship with joint inflammation." dans: The Journal of rheumatology, Vol. 38, Issue 9, pp. 1966-72, (2011) (PubMed).

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: GAPDH