RNASE1 anticorps (HRP)

N° du produit ABIN5596978

N° du produit (Fournisseur): 200-4388-0100

Cet anticorps anti-RNASE1 est un anticorps Lapin Polyclonal détectant RNASE1 dans WB, IP et ELISA. Adapté pour Boeuf (Vache).

Aperçu rapide pour RNASE1 anticorps (HRP) (ABIN5596978)

Antigène: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RNASE1 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Détail du produit anti-RNASE1 anticorps (HRP)

N° du produit (Fournisseur): 200-4388-0100

Fournisseur: Rockland

Fonction: Ribonuclease A Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine Pancreas].

Attributs du produit: Synonyms: rabbit anti-Ribonuclease A Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Ribonuclease A Antibody, Pancreatic ribonuclease antibody, Rib 1 antibody, Rib1 antibody, Ribonuclease a family 1 antibody, Ribonuclease pancreatic antibody, Ribonuclease RNase A family 1 antibody, Ribonuclease RNase A family 1 pancreatic antibody

Purification: Ribonuclease A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Ribonuclease A [Bovine Pancreas]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Ribonuclease A Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Ribonuclease A [Bovine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:40,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur RNASE1

Antigène: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Autre désignation: RNASE1

Sujet: Background: Ribonuclease A is a pancreatic ribonuclease that cleaves single-stranded RNA. Bovine pancreatic RNase A is one of the classic model systems of protein science. RNase A cleaves specifically after pyrimidine nucleotides. It can be inhibited by ribonuclease inhibitor protein, by heavy metal ions, and by uridine-vanadate complexes. RNase A, and to a greater extent its oligomers and some homologs, have cytotoxic and cytostatic effects, particularly on cancer cells.

ID gène: 282340

UniProt: P61823