SMAD3 anticorps (pThr179)

N° du produit ABIN5608745

Cet anticorps Lapin Polyclonal détecte spécifiquement SMAD3 dans WB, ELISA, IHC (p), FACS, ICC, IF (cc), IF (p) et IHC (fro). Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 1 publication.

Aperçu rapide pour SMAD3 anticorps (pThr179) (ABIN5608745)

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-SMAD3 anticorps

Épitope: pThr179

Réactivité croisée: Humain, Souris, Rat

Homologie: Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Thr179

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-SMAD3 (ABIN5608745)

Zhang, Ju, Zhang, Zhu, Nie, Xu, Lei et al.: "Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4(+) T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 ..." dans: Basic & clinical pharmacology & toxicology, Vol. 120, Issue 6, pp. 560-570, (2016) (PubMed).

Détails sur SMAD3

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Autre désignation: Smad3

Sujet:

Synonyms: Mothers against decapentaplegic homolog 3, SMAD3, JV15-2

Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

ID gène: 4088

UniProt: P84022

Pathways: Cycle Cellulaire, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy