beta Actin anticorps (N-Term)

N° du produit ABIN5611427

L’anticorps Lapin Polyclonal anti-beta Actin a été validé pour WB et IHC (p). Il convient pour détecter beta Actin dans des échantillons de Humain, Souris, Rat, Poulet, Lapin, Chien, Porc, Boeuf (Vache), Mouton, Cobaye et Cat.

Aperçu rapide pour beta Actin anticorps (N-Term) (ABIN5611427)

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain, Souris, Rat, Poulet, Lapin, Chien, Porc, Boeuf (Vache), Mouton, Cobaye, Cat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp beta Actin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-beta Actin anticorps

Épitope: N-Term

Specificité: This antibody recognizes Actin-Beta (ACTB).

Réactivité croisée (Details): Species reactivity (expected):Bovine, Cat, Chicken, Canine (Dog), Guinea Pig, Mouse, Porcine, Rabbit, Rat and Sheep.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogène: N-terminal Human beta Actin.

Information d'application

Indications d'application: Western blot. Immunohistochemistry on Paraffin Sections: 10 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by Alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: 10 mM PBS, pH 7.4 containing BSA and Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: Actin beta / ACTB

Sujet: Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. The two major cytoskeletal proteins implicated in cell motility are actin and myosin. There are three alpha-actins (skeletal, cardiac, and smooth muscle), one beta actin (non-muscle), and two gamma-actins (gamma-smooth muscle and gamma-nonmuscle). Antibody to beta-actin provides a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Actin is a highly conserved protein that participates in a wide variety of cellular functions in eukaryotes including muscle contraction, amoeboid movement, cytokinesis and mitotic division. Beta actin is ubiquitously expressed in all eukaryotic cells.Synonyms: Actin cytoplasmic 1, Beta-Actin

ID gène: 60

NCBI Accession: NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction