Recombinant Polysialic Acid anticorps

N° du produit ABIN5668052

Cité dans 1 publication. Cet anticorps anti-Polysialic Acid Monoclonal Souris (Clone 735) (ABIN5668052) détecte spécifiquement Polysialic Acid dans FACS, IHC, IP et WB. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Recombinant Polysialic Acid anticorps (ABIN5668052)

Antigène: Polysialic Acid

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Clone: 735

Détail du produit

Fonction: Anti-Polysialic acid [735], Mouse IgG2a, kappa

Specificité: Recognises Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of α2,8-linked sialic acid of Escherichia coli K1.

Réactivité croisée: Souris, Rat

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: Homopolymers of alpha2,8-linked sialic acid of Escherichia coli K1.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Clone 735 was used for immunostaining and immunoprecipitation in an experimental setup involving antigen preparation from PBMC (Peripheral Blood Mononuclear Cells) (Husmann et al., 1989, PMID: 2529126). This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al., 1990). 735 was used for Western blot analysis of PSA-NCAM (Polysialylated Neural Cell Adhesion Molecule) (Charter et al., 2000, PMID: 11030751). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al., 2006).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Référence

Tytgat, Lin, Levasseur, Tomek, Rutschmann, Mock, Liebscher, Terasaka, Azuma, Wetter, Bachmann, Hilvert, Aebi, Keys: "Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies." dans: Nature communications, Vol. 10, Issue 1, pp. 5403, (2020) (PubMed).

Détail du antigène

Antigène: Polysialic Acid

Sujet: PSA, N-CAM, neural cell adhesion molecule, polySA