Collagen IV anticorps

N° du produit ABIN5680242

Cet anticorps Souris Monoclonal détecte spécifiquement Collagen IV dans WB, IHC (fro) et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour Collagen IV anticorps (ABIN5680242)

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Collagen IV est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: COL 94

Détail du produit anti-Collagen IV anticorps

Specificité: Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate Human and other primates, no reactivity in chicken

Aucune reactivité croisée: Cat, Poulet, Chévre, Lapin, Rat, Mouton

Réactivité croisée (Details): Human and other primates (no reactivity in chicken, cat, rabbit, rat, sheep and goat)

Attributs du produit: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate.

Purification: Antibody solution

Immunogène: Purified human Collagen IV

Isotype: IgG1

Information d'application

Indications d'application: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human skin, placenta or lens capsule,IHC(C,P), WB,60 min at RT,(liquid conc.) 1:10 - 1:50

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: stabilizing phosphate buffer  pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 μL working solution / test)

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur Collagen IV

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Autre désignation: Collagen Type IV