Collagen IV anticorps

N° du produit ABIN5680248

Cet anticorps Lapin Polyclonal détecte spécifiquement Collagen IV dans WB, ELISA, IHC (p), IF et RIA. Il présente une réactivité envers Souris.

Aperçu rapide pour Collagen IV anticorps (ABIN5680248)

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen IV est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Détail du produit anti-Collagen IV anticorps

Specificité: Murine collagen type IV 100 % , murine collagen type I, II and III <0.1 %, human collagen type IV and V <0.1 %, murine fibronectin <0.1 %, murine laminin <0.1 % in RIA at 1:5000 dilution

Réactivité croisée (Details): Mouse

Attributs du produit: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Murine collagen type IV 100 % , murine collagen type I, II and III <0.1 %, human collagen type IV and V <0.1 %, murine fibronectin <0.1 %, murine laminin <0.1 % in RIA at 1:5000 dilution.

Purification: Affinity purified

Immunogène: Purified collagen type IV from murine tumour tissue

Isotype: IgG

Information d'application

Indications d'application: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Mouse skin or liver,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Agent conservateur: Without preservative

Stock: -20 °C

Détails sur Collagen IV

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Autre désignation: Collagen Type IV