Glucuronidase beta anticorps (Biotin)

N° du produit ABIN568042

Cet anticorps Lapin Polyclonal détecte spécifiquement Glucuronidase beta dans WB, IP, IF, ID, EIA, RIA et DB. Il présente une réactivité avec des échantillons de E. coli.

Aperçu rapide pour Glucuronidase beta anticorps (Biotin) (ABIN568042)

Antigène: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Glucuronidase beta est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB)

Détail du produit anti-Glucuronidase beta anticorps (Biotin)

Specificité: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Attributs du produit: Molar Ratio: Biotin/IgG~ 6.2

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogène: Beta-Glucuronidase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water.

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Détails sur Glucuronidase beta

Antigène: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Autre désignation: beta-Glucuronidase

Sujet: Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.Synonyms: Beta-G1, GUSB

ID gène: 946149

NCBI Accession: AP_002238

UniProt: P05804

Pathways: Glycosaminoglycan Metabolic Process