GAPDH anticorps

N° du produit ABIN568230

Cet anticorps anti-GAPDH est un anticorps Mouton Polyclonal détectant GAPDH dans WB, IF, IP, EIA, DB, RIA et ID. Adapté pour Lapin.

Aperçu rapide pour GAPDH anticorps (ABIN568230)

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Lapin

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Radioimmunoassay (RIA), Immunodiffusion (ID)

Détail du produit anti-GAPDH anticorps

Specificité: The antibody recognizes GAPDH from Rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Purification: Solid Phase Affinity Chromatography

Immunogène: Glyceraldehyde-3-Phosphate Dehydrogenase isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 0.5 mL of sterile distilled water

Concentration: 2.0 mg/mL

Buffer: PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: GAPDH

Sujet: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase

ID gène: 100009074

NCBI Accession: NP_001075722

UniProt: P46406