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Lactate Dehydrogenase A anticorps (Biotin)

L’anticorps Mouton Polyclonal anti-Lactate Dehydrogenase A a été validé pour WB, IP, IF, EIA, DB, ID et RIA. Il convient pour détecter Lactate Dehydrogenase A dans des échantillons de Lapin.
N° du produit ABIN568298

Aperçu rapide pour Lactate Dehydrogenase A anticorps (Biotin) (ABIN568298)

Antigène

Voir toutes Lactate Dehydrogenase A (LDHA) Anticorps
Lactate Dehydrogenase A (LDHA)

Reactivité

  • 82
  • 37
  • 28
  • 10
  • 7
  • 6
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Lapin

Hôte

  • 90
  • 21
  • 6
  • 4
Mouton

Clonalité

  • 97
  • 22
Polyclonal

Conjugué

  • 61
  • 16
  • 13
  • 9
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
Cet anticorp Lactate Dehydrogenase A est conjugé à/à la Biotin

Application

  • 85
  • 47
  • 44
  • 31
  • 23
  • 22
  • 10
  • 9
  • 5
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)
  • Specificité

    L-Lactic Dehydrogenase from Rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

    Attributs du produit

    Molar Ratio: Biotin/IgG ~ 6.6

    Purification

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography

    Immunogène

    L-Lactic Dehydrogenase is isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Reconstitution

    Restore by adding 1.0 mL of sterile distilled water

    Concentration

    10 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins

    Agent conservateur

    Without preservative

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Antigène

    Lactate Dehydrogenase A (LDHA)

    Autre désignation

    LDHA

    Sujet

    Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).Synonyms: Cell proliferation-inducing gene 19 protein, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, NY-REN-59, PIG19, Renal carcinoma antigen NY-REN-59

    ID gène

    100009107

    NCBI Accession

    NP_001075746

    UniProt

    P13491

    Pathways

    L'effet Warburg
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