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Alkaline Phosphatase anticorps

Cet anticorps anti-Alkaline Phosphatase est un anticorps Lapin Polyclonal détectant Alkaline Phosphatase dans WB, IF, IP, EIA, ID, RIA et DB. Adapté pour E. coli.
N° du produit ABIN568362

Aperçu rapide pour Alkaline Phosphatase anticorps (ABIN568362)

Antigène

Voir toutes Alkaline Phosphatase (ALP) Anticorps
Alkaline Phosphatase (ALP)

Reactivité

  • 70
  • 18
  • 13
  • 11
  • 8
  • 1
  • 1
E. coli

Hôte

  • 59
  • 28
  • 2
  • 1
  • 1
Lapin

Clonalité

  • 47
  • 44
Polyclonal

Conjugué

  • 45
  • 11
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Cet anticorp Alkaline Phosphatase est non-conjugé

Application

  • 52
  • 31
  • 22
  • 16
  • 12
  • 10
  • 8
  • 7
  • 7
  • 5
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB)
  • Specificité

    Alkaline Phosphatase from Escherichia coli. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

    Purification

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography

    Immunogène

    Alkaline Phosphatase isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore by adding 1.0 mL of sterile distilled water

    Concentration

    10.0 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins

    Agent conservateur

    Without preservative

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Antigène

    Alkaline Phosphatase (ALP)

    Autre désignation

    Alkaline Phosphatase

    Sujet

    Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.Synonyms: JW0374, b0383, phoA

    ID gène

    945041

    NCBI Accession

    AP_001034

    UniProt

    P00634
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