IL-2 anticorps (Biotin)

N° du produit ABIN5687588

Cet anticorps anti-IL-2 est un anticorps Chèvre Polyclonal détectant IL-2 dans WB et ELISA. Adapté pour Humain.

Aperçu rapide pour IL-2 anticorps (Biotin) (ABIN5687588)

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-2 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-IL-2 anticorps (Biotin)

Purification: Anti-hIL-2 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogène: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-2 (human IL-2).

Information d'application

Indications d'application: ELISA:

To detect hIL-2 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-2.

Sandwich:

To detect hIL-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-2 (XP-5182) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-2.

Western Blot:

To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: IL-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur IL-2

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Autre désignation: IL-2

Sujet: Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.

ID gène: 3558

UniProt: P60568

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation