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Thrombopoietin anticorps (Biotin)

Cet anticorps anti-Thrombopoietin est un anticorps Chèvre Polyclonal détectant Thrombopoietin dans WB et ELISA. Adapté pour Humain.
N° du produit ABIN5687618

Aperçu rapide pour Thrombopoietin anticorps (Biotin) (ABIN5687618)

Antigène

Voir toutes Thrombopoietin (THPO) Anticorps
Thrombopoietin (THPO)

Reactivité

  • 43
  • 14
  • 11
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Humain

Hôte

  • 42
  • 14
  • 6
  • 5
Chèvre

Clonalité

  • 54
  • 13
Polyclonal

Conjugué

  • 51
  • 6
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Thrombopoietin est conjugé à/à la Biotin

Application

  • 52
  • 27
  • 17
  • 15
  • 14
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA
  • Purification

    Anti-hTPO specific antibody was purified by affinity chromatography and then biotinylated.

    Immunogène

    Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTPO (human TPO).
  • Indications d'application

    ELISA:

    To detect hTPO by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hTPO.

    Sandwich:

    To detect hTPO by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TPO (XP-5288) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.

    Western Blot:
    To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Stock

    -20 °C

    Stockage commentaire

    TPO antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • Antigène

    Thrombopoietin (THPO)

    Autre désignation

    Thrombopoietin (TPO)

    Sujet

    Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

    ID gène

    7066

    UniProt

    P40225

    Pathways

    Signalistation JAK/STAT, Hormone Activity
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