Thrombopoietin anticorps

N° du produit ABIN5687619

Cet anticorps Chèvre Polyclonal détecte spécifiquement Thrombopoietin dans WB, ELISA et Neut. Il présente une réactivité envers Humain.

Aperçu rapide pour Thrombopoietin anticorps (ABIN5687619)

Antigène: Thrombopoietin (THPO)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Thrombopoietin est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-Thrombopoietin anticorps

Purification: Anti-hTPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix.

Immunogène: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTPO.

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTPO (5 ng/mL), a concentration of 4.2 - 6.3 μ,g/mL of this antibody is required.

ELISA:

To detect hTPO by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTPO.

Sandwich:

To detect hTPO by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TPO (XP-5288Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.

Western Blot:
To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: TPO antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur Thrombopoietin

Antigène: Thrombopoietin (THPO)

Autre désignation: Thrombopoietin (TPO)

Sujet: Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

ID gène: 7066

UniProt: P40225

Pathways: Signalistation JAK/STAT, Hormone Activity