Cet anticorps Lapin Polyclonal détecte spécifiquement Synaptotagmin dans WB et IHC. Il présente une réactivité avec des échantillons de Rat, Souris et Humain.
Synaptotagmin antibody is specific for Mr 60 - 62k synaptotagmin phosphorylated at Ser309.
Purification
Affinity Purified
Immunogène
Synaptotagmin (Ser309) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Ser309 of synaptotagmin.
SYT
Reactivité: Rat, Souris
WB, ELISA, IHC (p), IHC (fro)
Hôte: Lapin
Polyclonal
Biotin
Indications d'application
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, and rat have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Restrictions
For Research Use only
Format
Liquid
Buffer
100 μLin 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.
Stock
-20 °C
Stockage commentaire
For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Antigène
Synaptotagmin (SYT)
Autre désignation
Synaptotagmin
Sujet
Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis. Moreover recent studies indicate that the protein also plays a key role in endocytosis. Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission.