CAMK2A anticorps (pThr286)

N° du produit ABIN5688927

Cet anticorps Lapin Polyclonal détecte spécifiquement CAMK2A dans WB. Il présente une réactivité envers Humain, Souris, Rat et Xenopus laevis.

Aperçu rapide pour CAMK2A anticorps (pThr286) (ABIN5688927)

Antigène: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivité: Humain, Souris, Rat, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CAMK2A est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-CAMK2A anticorps

Épitope: pThr286

Specificité: CaM Kinase II antibody is specific for a 50 kDa and b 60 kDa CaM Kinase II phosphorylated at Thr286.

Purification: Affinity Purified

Immunogène: CaM Kinase II (Thr286) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.

Information d'application

Indications d'application: Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of rat brain lysates with phospho- and dephosphopeptide competition were used to establish the phospho-specificity of the antibody. Applications include Dot Blot (DB) and Western Blot (WB). Dot Blots and Western Blots performed with each lot and working dilutions internally tested under ideal conditions for DB and WB were 1:1000. Suitability for Immunohistochemistry (IHC) has not been determined. Human, mouse, rat and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Stock: -20 °C

Stockage commentaire: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Détails sur CAMK2A

Antigène: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Autre désignation: CaM Kinase II

Sujet: Calcium/calmodulin kinase II (CaM Kinase II) is multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to second messengers. Phosphorylation of Thr286 produces an autonomously active form of CaM Kinase II. The antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.

Poids moléculaire: 50/60 kDa

ID gène: 25400

UniProt: P11275

Pathways: Signalisation WNT, Interferon-gamma Pathway, Myometrial Relaxation and Contraction