IL1A anticorps
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- Antigène Voir toutes IL1A Anticorps
- IL1A (Interleukin 1 alpha (IL1A))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp IL1A est non-conjugé
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Purification
- Anti-hIL-1alpha specific antibody was purified by affinity chromatography employing immobilized hIL-1alpha matrix.
- Immunogène
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-1alpha (human Interleukin-1-alpha).
- Top Product
- Discover our top product IL1A Anticorps primaire
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- Indications d'application
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1α, (20 ng/mL), a concentration of 1.0 - 2.0 μ,g/mL of this antibody is required.
ELISA:
To detect hIL-1α, by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-1α,.
Sandwich:
To detect hIL-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-1α, (XP-5174Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-1α,.
Western Blot:
To detect hIL-1α, by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1α, is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Stock
- -20 °C
- Stockage commentaire
- IL-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Antigène
- IL1A (Interleukin 1 alpha (IL1A))
- Autre désignation
- IL-1a (IL1A Produits)
- Synonymes
- anticorps IL-1A, anticorps IL1, anticorps IL1-ALPHA, anticorps IL1F1, anticorps IL-6, anticorps Il-1a, anticorps IL-1 alpha, anticorps IL-1alpha, anticorps L1A, anticorps interleukin 1 alpha, anticorps interleukin 6, anticorps IL1A, anticorps IL6, anticorps Il1a
- ID gène
- 3552
- UniProt
- P01583
- Pathways
- Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints
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