CXCL12 anticorps

N° du produit ABIN5689108

L’anticorps Lapin Polyclonal anti-CXCL12 a été validé pour WB, ELISA et Neut. Il convient pour détecter CXCL12 dans des échantillons de Humain.

Aperçu rapide pour CXCL12 anticorps (ABIN5689108)

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CXCL12 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-CXCL12 anticorps

Purification: Anti-hSDF-1-alpha specific antibody was purified by affinity chromatography employing immobilized hSDF-1-alpha matrix.

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hSDF-1-alpha (human Stromal Cell-Derived Factor-1-alpha).

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hSDF1α, (100 ng/mL), a concentration of 2-4 μ,g/mL of this antibody is required.

ELISA:

To detect hSDF-1-alpha by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hSDF-1-alpha.

Sandwich:

To detect hSDF-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human SDF-1α, (XP-5269Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSDF-1α,.

Western Blot:
To detect hSDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: SDF-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur CXCL12

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Autre désignation: SDF-1a

ID gène: 6387

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling