GFI1B anticorps (AA 322-326)

N° du produit ABIN5689567

L’anticorps anti-GFI1B Polyclonal Lapin est utilisé pour la détection de GFI1B dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB.

Aperçu rapide pour GFI1B anticorps (AA 322-326) (ABIN5689567)

Antigène: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GFI1B est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-GFI1B anticorps

Épitope: AA 322-326

Specificité: This antibody detects endogenous level of total GFI1b protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogène: GFI1b antibody  was raised against a peptide sequence around aa.322~326 (H-R-E-S-Q) derived from Human GFI1b.

Information d'application

Indications d'application: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store antibody at -20°C for up to one year.

Détails sur GFI1B

Antigène: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Autre désignation: GFI1b

Sujet: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context, represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1, GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription, as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.

Poids moléculaire: 38 kDa

ID gène: 8328

NCBI Accession: NP_001128503

UniProt: Q5VTD9

Pathways: Cellular Response to Molecule of Bacterial Origin