This antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Immunogène
MARCKS (Phospho-Ser162) antibody was raised against a peptide sequence around phosphorylation site of serine 162 (K-K-S (p) -F-K) derived from Human MARCKS.
MARCKS
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
RB04646
unconjugated
Indications d'application
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Store antibody at -20°C for up to one year.
Antigène
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
Autre désignation
MARCKS
Sujet
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.