Rubicon anticorps (AA 240-290)

N° du produit ABIN5690236

Détail du produit anti-Rubicon anticorps

Antigène: Rubicon (KIAA0226) (KIAA0226)

Épitope: AA 240-290

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Rubicon est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.

Purification: Rubicon antibody is affinity chromatography purified via peptide column.

Immunogène: Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.

The immunogen is located within amino acids 240 - 290 of Rubicon.

Isotype: IgG

Information d'application

Indications d'application: Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rubicon antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Rubicon antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Détails sur Rubicon

Antigène: Rubicon (KIAA0226) (KIAA0226)

Autre désignation: Rubicon (KIAA0226 Produits)

Synonymes: anticorps RUBICON, anticorps 5330403K09, anticorps Kiaa0226, anticorps Rubicon, anticorps mKIAA0226, anticorps RUN and cysteine rich domain containing beclin 1 interacting protein, anticorps KIAA0226, anticorps RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein, anticorps RUBCN, anticorps KIAA0226, anticorps Rubcn

Sujet: Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).

Poids moléculaire: Predicted: 90, 101, 109 kDa

Observed: 91, 99 kDa

ID gène: 9711

NCBI Accession: XP_005269431

UniProt: Q92622

Pathways: Autophagy