GM-CSF anticorps (Biotin)

N° du produit ABIN5690566

L’anticorps anti-GM-CSF Polyclonal Lapin est utilisé pour la détection de GM-CSF dans des échantillons de Rat. Il a été validé pour WB et ELISA.

Aperçu rapide pour GM-CSF anticorps (Biotin) (ABIN5690566)

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GM-CSF est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-GM-CSF anticorps (Biotin)

Immunogène: Produced from sera of rabbits immunized with highly pure recombinant Rat GM-CSF. Rat GM-CSF specific antibody was purified by affinity chromatography and then biotinylated.

Information d'application

Indications d'application: ELISA:
Direct:
To detect Rat GM-CSF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.

Sandwich
To detect Rat GM-CSF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci's Polyclonal Anti-Rat GM-CSF (38-273) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.

Western Blot:

To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur GM-CSF

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Autre désignation: GM-CSF

Sujet: GM-CSF is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors.

ID gène: 116630

UniProt: P48750

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin