ApoE3 anticorps (Biotin)

N° du produit ABIN5690605

L’anticorps anti-ApoE3 Polyclonal Lapin est utilisé pour la détection de ApoE3 dans des échantillons de Humain. Il a été validé pour ELISA et WB.

Aperçu rapide pour ApoE3 anticorps (Biotin) (ABIN5690605)

Antigène: ApoE3 (Apolipoprotein E3 (ApoE3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ApoE3 est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB)

Détail du produit anti-ApoE3 anticorps (Biotin)

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hApoE3. Human ApoE3 specific antibody was purified by affinity chromatography and then biotinylated. *Cross Reactivity: human ApoE2, human ApoE4 in both western blot and sandwich ELISA

Information d'application

Indications d'application: ELISA:
Direct:
To detect hApoE3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.

Sandwich
To detect hApoE3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human ApoE3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.

Western Blot:
To detect hApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hApoE3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: ApoE3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur ApoE3

Antigène: ApoE3 (Apolipoprotein E3 (ApoE3))

Autre désignation: ApoE3

ID gène: 348

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism