Perforin 1 anticorps (AA 98-534)

N° du produit ABIN5690663

Cet anticorps anti-Perforin 1 est un anticorps Rat Monoclonal détectant Perforin 1 dans WB, IHC, IP et ICC. Adapté pour Souris.

Aperçu rapide pour Perforin 1 anticorps (AA 98-534) (ABIN5690663)

Antigène: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Perforin 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: CB5-4

Détail du produit anti-Perforin 1 anticorps

Épitope: AA 98-534

Purification: Perforin (monoclonal CB5.4) antibody was purified to ≥,95% in SDS-PAGE.

Immunogène: Perforin (monoclonal CB5.4) antibody was raised against a recombinant mouse perforin, amino acids 98-534.

Isotype: IgG2a

Information d'application

Indications d'application: Perforin antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1 % BSA, slides were incubated with 5 μ,g/mL of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC). Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 μ,g/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Perforin antibody can be stored at 4°C, stable for one year.

Détails sur Perforin 1

Antigène: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Autre désignation: Perforin

ID gène: 18646

UniProt: P10820

Pathways: Apoptose, Caspase Cascade in Apoptosis