Tryptophan Hydroxylase 1 anticorps

N° du produit ABIN5690709

Cet anticorps Mouton Polyclonal détecte spécifiquement Tryptophan Hydroxylase 1 dans WB et IHC. Il présente une réactivité envers Rat.

Aperçu rapide pour Tryptophan Hydroxylase 1 anticorps (ABIN5690709)

Antigène: Tryptophan Hydroxylase 1 (TPH1)

Reactivité: Rat

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Tryptophan Hydroxylase 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-Tryptophan Hydroxylase 1 anticorps

Purification: Affinity Purified

Immunogène: TPH polyclonal antibody was raised against recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E.

Information d'application

Indications d'application: Tryptophan Hydroxylase antibody can be used for specific immunolabeling of the ~55k TPH protein in Western blots of human dorsal Raphe nucleus. Applications include Dot Blots (DB) Immunohistochemistry (IHC) and Western Blots (WB). The antibody has been used successfully with rat and human brain tissues in blot immunolabeling and immunohistochemical procedures. Based upon the relatively high degree of homology of tryptophan hydroxylase, the antibodies should cross-react with other mammalian species. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Stock: -20 °C

Stockage commentaire: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Détails sur Tryptophan Hydroxylase 1

Antigène: Tryptophan Hydroxylase 1 (TPH1)

Autre désignation: Tryptophan Hydroxylase

Sujet: Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis..

Poids moléculaire: 55 kDa

ID gène: 100009100

UniProt: P17290

Pathways: Feeding Behaviour