TNC anticorps

N° du produit ABIN5691113

Détail du produit anti-TNC anticorps

Antigène: TNC (Tenascin C (TNC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNC est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: In immunoblotting assays on an extract of a human osteosarcoma cell line (U2OS) T2H5 recognizes a 265 kDa protein. Tenascin C contains an exstensive number of potential glycosylation sites, which may explain its migration at a higher molecular weight than predicted. Human tonsilar tissue can be used as a positive control in immunocytochemistry and immunohistochemistry.

Immunogène: T2H5 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a BALB/c mouse immunized with full length native tenascin protein purified from a homogenate of a human mammary tumour specimen.

Clone: T2H5

Isotype: IgG1

Information d'application

Indications d'application: T2H5 is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Buffer: Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C, or in small aliquots at -20°C.

Détails sur TNC

Antigène: TNC (Tenascin C (TNC))

Autre désignation: Tenascin C (TNC Produits)

Synonymes: anticorps tn, anticorps 150-225, anticorps GMEM, anticorps GP, anticorps HXB, anticorps JI, anticorps TN, anticorps TN-C, anticorps AI528729, anticorps C130033P17Rik, anticorps Hxb, anticorps Ten, anticorps cytotactin, anticorps tenascin-C, anticorps P230, anticorps tenc, anticorps wu:fk04d02, anticorps tenascin C L homeolog, anticorps tenascin C, anticorps tnc.L, anticorps TNC, anticorps MCYG_00667, anticorps tnc, anticorps Tnc

Sujet: Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development. It has been described under a variety of names, i.e. cytotactin, hexabrachion protein, J1, myotendinous antigen and glioma mesenchymal extracellular matrix. In undifferentiated tumors it is also detectable in the neovasculature and stroma. The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. For example, this extracellular matrix protein is implicated in guidance of migrating neurons as well as axons during neural development as well as neuronal regeneration. In in vitro studies it promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. The cellular ligands for tenascin include integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development