RNF43 anticorps (AA 24-197)

N° du produit ABIN5693038

L’anticorps Lapin Polyclonal anti-RNF43 a été validé pour ELISA et WB. Il convient pour détecter RNF43 dans des échantillons de Humain.

Aperçu rapide pour RNF43 anticorps (AA 24-197) (ABIN5693038)

Antigène: RNF43 (Ring Finger Protein 43 (RNF43))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RNF43 est non-conjugé

Application: ELISA, Western Blotting (WB)

Détail du produit anti-RNF43 anticorps

Épitope: AA 24-197

Fonction: Anti-RNF43 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-RNF43 Antibody Picoband® (ABIN5693038). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogène: E. coli-derived human RNF43 recombinant protein (Position: G24-Y197).

Isotype: IgG

Information d'application

Indications d'application: Western blot,0.1-0.5 μg/mL
ELISA,0.1-0.5 μg/mL
1. Sugiura, T., Yamaguchi, A., Miyamoto, K. A cancer-associated RING finger protein, RNF43, is a ubiquitin ligase that interacts with a nuclear protein, HAP95. Exp. Cell Res. 314: 1519-1528, 2008. 2. Yagyu, R., Furukawa, Y., Lin, Y.-M., Shimokawa, T., Yamamura, T., Nakamura, Y. A novel oncoprotein RNF43 functions in an autocrine manner in colorectal cancer. Int. J. Oncol. 25: 1343-1348, 2004.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur RNF43

Antigène: RNF43 (Ring Finger Protein 43 (RNF43))

Autre désignation: RNF43

Sujet:

Synonyms: E3 ubiquitin-protein ligase RNF43, RING finger protein 43, RING-type E3 ubiquitin transferase RNF43, RNF43

Tissue Specificity: Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines.

Background: RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.

Poids moléculaire: 86 kDa

ID gène: 54894

Pathways: Signalisation WNT