PKM1 anticorps

N° du produit ABIN5697233

Détail du produit

Antigène: PKM1

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF)

Immunogène: PKM2 pyruvate kinase, muscle

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with FNab06494(PKM1-specific) at dilution of 1:1500

Restrictions: For Research Use only

Stockage

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Date de péremption: 12 months

Détail du antigène

Antigène: PKM1

Sujet: Synonyms:CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK Background:PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate(PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. By the upstream metabolite fructose-1,6-bisphosphate, PKM1 is a constitutively active enzyme.The primary pyruvate kinase isoform before tumour development is PK-M1, however, the primary isoform from four independent tumours is PK-M2(PMID:18337823). This antibody is specific to PKM1 isoform.