Histone 3 anticorps (H3K36me3)

N° du produit ABIN5706765

N° du produit (Fournisseur): 600-401-i88

Cet anticorps anti-Histone 3 est un anticorps Lapin Polyclonal détectant Histone 3 dans WB, IHC, IF, DB, FM et MA. Adapté pour Humain, Souris et C. elegans.

Aperçu rapide pour Histone 3 anticorps (H3K36me3) (ABIN5706765)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, Souris, C. elegans

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Dot Blot (DB), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Détail du produit anti-Histone 3 anticorps

Épitope: H3K36me3

N° du produit (Fournisseur): 600-401-i88

Fournisseur: Rockland

Fonction: Histone H3 K36me3 Antibody

Réactivité croisée (Details): This antibody reacts with human Histone H3.

Attributs du produit: Synonyms: rabbit anti-Histone H3 trimethyl Lys36 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K36me3

Purification: Anti-Histone H3 [Trimethyl Lys36] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Histone H3 [Trimethyl Lys36] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 36 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-Histone H3 [Trimethyl Lys36] antibody is tested for Western Blot, Dot Blot, and Immunofluorescence. This antibody is useful in Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:500

Other: Dot Blot 0.5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Background: The histone modification H3K36me3 is important in the differentiation and maintenance of specialized cells derived from stem cell progenitors. The presence and abundance of H3K36me3 is correlated to the downstream transcription of those pathway components important for cellular functions that differentiate cells from each other. The polycomb repressive complex 2 (PRC2) represses transcription by methylation of H3 lysine 27, but this methylation is inhibited by the presence of the H3K36me3 mark. SETD2 is the main methyltransferase responsible for methylating the H3K36me3. Once methylated, H3K36me3 is highly associated with active transcription factors, and the generation of integral downstream pathways, which move stem cells towards differentiation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

ID gène: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3