Histone 3 anticorps (H3K4me3, pThr6)

N° du produit ABIN5706775

N° du produit (Fournisseur): 600-401-i62

Cet anticorps Lapin Polyclonal détecte spécifiquement Histone 3 dans WB, IHC, IF, ChIP, DB, FM et MA. Il présente une réactivité envers Humain et C. elegans.

Aperçu rapide pour Histone 3 anticorps (H3K4me3, pThr6) (ABIN5706775)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, C. elegans

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Détail du produit anti-Histone 3 anticorps

Épitope: H3K4me3, pThr6

N° du produit (Fournisseur): 600-401-i62

Fournisseur: Rockland

Fonction: Histone H3 K4me3/phospho T6 Antibody

Réactivité croisée (Details): This antibody reacts with human Histone H3.

Attributs du produit: Synonyms: rabbit anti-Histone H3 trimethyl Lys4 pT6 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4me3/pT6

Purification: Anti-Histone H3 [Trimethyl Lys4, p Thr6] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Histone H3 [Trimethyl Lys4, p Thr6] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic trimethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 6 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50

Application Note: Anti-Histone H3 [Trimethyl Lys4, p Thr6] antibody is tested for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:50

Other: Dot Blot 0.5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.68 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Background: Phosphorylation at T6 of methylated H3K4 prevents LSD1 from demethylating histone H3. Androgen receptor activated gene expression depends upon removal of methyl groups from H3K4, in cooperation with the Jumonji protein JMJD2C. However, when T6 is phosphorylated, there is a physical obstruction in the way of demethylation, and thus gene expression is repressed. The PHD finger of H3K4 seems to be an effector of histone modification, which can cause dysfunction in cellular fate regulation. Interestingly, the abundance of phosphorylation of this modified histone is a probable biomarker for the detection and the prognosis of certain cancers. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

ID gène: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3