Histone 3 anticorps (H3K9ac)

N° du produit ABIN5706781

N° du produit (Fournisseur): 600-401-i72

Cet anticorps Lapin Polyclonal détecte spécifiquement Histone 3 dans WB, IHC, IF, ChIP, DB et FM. Il présente une réactivité envers Humain, Souris et C. elegans et a été mentionné dans 1 publication.

Aperçu rapide pour Histone 3 anticorps (H3K9ac) (ABIN5706781)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, Souris, C. elegans

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)

Détail du produit anti-Histone 3 anticorps

Épitope: H3K9ac

N° du produit (Fournisseur): 600-401-i72

Fournisseur: Rockland

Fonction: Histone H3 K9ac Antibody

Réactivité croisée (Details): This antibody reacts with human Histone H3.

Attributs du produit: Synonyms: rabbit anti-Histone H3 Ac Lys9 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9ac

Purification: Anti-Histone H3 [ac Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Histone H3 [ac Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 9 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-Histone H3 [ac Lys9] antibody is tested for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:100

Other: Dot Blot 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.68 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-Histone 3 (ABIN5706781)

Moiseeva, Hood, Schamus, OConnor, Conrads, Bakkenist: "ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1." dans: Nature communications, Vol. 8, Issue 1, pp. 1392, (2018) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Background: Acetylation of histone H3 at the K9 residue is associated with chromosome condensation in mitotic cells. The presence of H3K9ac is correlated to H3K4 trimethylation, and their distribution is related to developmentally repressed genes in some species. It has been shown that neuron depolarization promotes intragenic histone acetylation leading toward the formation of this modification. In pituitary, dopamine receptor agonists lead toward apoptosis. However, in tumors, decreased availability and activity of these agonists, combined with decreased H3K9ac leads toward resistance to chemotherapies and cancer survival and proliferation. Nuclear receptor PPAR ' induces the enrichment of H3K9ac enrichment on downstream promoters, which has effects on transcription and ultimately nuclear receptor transactivation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

ID gène: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3