Histone 3 anticorps (H3K4me, pThr6)

N° du produit ABIN5706876

N° du produit (Fournisseur): 600-401-i61

Cité dans 1 publication. Cet anticorps anti-Histone 3 Polyclonal Lapin (ABIN5706876) détecte spécifiquement Histone 3 dans WB, IHC, IF, ChIP, DB et FM. L’anticorps est réactif avec des échantillons de Humain et C. elegans.

Aperçu rapide pour Histone 3 anticorps (H3K4me, pThr6) (ABIN5706876)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, C. elegans

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)

Détail du produit anti-Histone 3 anticorps

Épitope: H3K4me, pThr6

N° du produit (Fournisseur): 600-401-i61

Fournisseur: Rockland

Fonction: Histone H3 K4me1/phospho T6 Antibody

Réactivité croisée (Details): This antibody reacts with human Histone H3.

Attributs du produit: Synonyms: rabbit anti-Histone H3 monomethyl Lys4 pT6 antibody, H3.3AH3F3H3F3B, H3.3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4me1/pT6

Purification: Anti-Histone H3 [Monomethyl Lys4, p Thr6] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Histone H3 [Monomethyl Lys4, p Thr6] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic monomethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 6 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50

Application Note: Anti-Histone H3 [Monomethyl Lys4, p Thr6] antibody is tested for Western Blot, Immunofluorescence, and Dot Blot. This antibody is useful for Immunocytochemistry and Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:50

Other: Dot Blot 2 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.55 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-Histone 3 (ABIN5706876)

Mika, Luelling, Pavek, Nartker, Heyneman, Jones, Barrott: "Epigenetic Changes at the Birc5 Promoter Induced by YM155 in Synovial Sarcoma." dans: Journal of clinical medicine, Vol. 8, Issue 3, (2019) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, trimethylation of lysine 4 on H3 (H3 K4Me3) is a well known mark of gene activation. However, the role of phosphorylation at threonine 6 on H3 (H3 pT6) is more obscure. Yet recently, the two modifications have been shown to interact with each other. When H3 T6 is phosphorylated by protein kinase C beta 1 (PRKCbeta), the histone demethylase LSD1 is prevented from removing methyl groups from H3 K4. This same study also correlated high levels of pT6 and PRKCbeta as a possible marker for prostate cancer, as well as tumor progression in xenografts. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

ID gène: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3