Histone 3 anticorps (H3R2me2s, pThr3)

N° du produit ABIN5706882

N° du produit (Fournisseur): 600-401-p14

Cet anticorps anti-Histone 3 Polyclonal Lapin (ABIN5706882) détecte spécifiquement Histone 3 dans WB, IF, DB et FM. L’anticorps est réactif avec des échantillons de Humain, Souris et Singe.

Aperçu rapide pour Histone 3 anticorps (H3R2me2s, pThr3) (ABIN5706882)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, Souris, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Fluorescence Microscopy (FM)

Détail du produit anti-Histone 3 anticorps

Épitope: H3R2me2s, pThr3

N° du produit (Fournisseur): 600-401-p14

Fournisseur: Rockland

Fonction: Histone H3 pT3/R2me2s Antibody

Réactivité croisée (Details): This antibody reacts with human Histone H3.

Attributs du produit: Synonyms: rabbit anti-Histone H3 pT3 Sym-dimethyl Arg2 antibody, Histone H3 pT3 dimethyl R2s, Histone H3 pT3/R2Me2s, Histone H3 antibodies

Purification: Anti-Histone H3 pT3/R2Me2s was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Histone H3 pT3/R2Me2s affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the pT3 and R2Me2s site of human Histone H3.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Histone H3 pT3/R2Me2s antibody is tested in Western Blot, Immunocytochemistry, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15 kDa corresponding to the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.21 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular phosphorylation of threonine 3 (H3 pT3) is a known mark of mitosis. Recent findings also demonstrate that pT3 can promote binding of survivin in the nucleosome. Arg2 may have opposing effects to pT3. Anti-Histone H3 pT3/R2Me2s is ideal for researchers Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

ID gène: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3