Histone 3 anticorps (pThr6)

N° du produit ABIN5706883

N° du produit (Fournisseur): 600-401-i63

Cet anticorps Lapin Polyclonal détecte spécifiquement Histone 3 dans WB, IHC, IF, ChIP, DB et FM. Il présente une réactivité envers Humain et C. elegans.

Aperçu rapide pour Histone 3 anticorps (pThr6) (ABIN5706883)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, C. elegans

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)

Détail du produit anti-Histone 3 anticorps

Épitope: pThr6

N° du produit (Fournisseur): 600-401-i63

Fournisseur: Rockland

Fonction: Histone H3 phospho T6 Antibody

Réactivité croisée (Details): This antibody reacts with human Histone H3.

Attributs du produit: Synonyms: rabbit anti-Histone H3 pT6 antibody, H3.3AH3F3H3F3B, H3.3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3pT6

Purification: Anti-Histone H3 [p Thr6] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Histone H3 [p Thr6] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding Threonine 6 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200

Application Note: Anti-Histone H3 [p Thr6] antibody is tested for Western Blot, Immunofluorescence, and Dot Blot. This antibody is useful in Chromatin Immunoprecipitation and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:200

Other: Dot Blot user optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.67 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Background: Phosphorylation as a post-translational modification on histone H3 modulates the ability of proteins to recognize and bind to the H3 tail. When additional modifications to H3 are present, in addition to the native T6 phosphorylation, there is an amplified effect. Several authors suggest that the complexity of these combined PTMs on histone tails should be described as a convoluted language, rather than as a strict code. Since the H3T6 phosphorylation seems to be constitutively present, its de-phosphorylation could be highly significant and affect transcription, repair and replication. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

ID gène: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3