SATB1 anticorps (Internal Region)

N° du produit ABIN571004

L’anticorps Chèvre Polyclonal anti-SATB1 a été validé pour WB, IHC, ELISA, IF et FACS. Il convient pour détecter SATB1 dans des échantillons de Humain.

Aperçu rapide pour SATB1 anticorps (Internal Region) (ABIN571004)

Antigène: SATB1 (SATB Homeobox 1 (SATB1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SATB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-SATB1 anticorps

Épitope: Internal Region

Fonction: SATB1

Séquence: ESDEENRQKT RPRT

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-ESDEENRQKTRPRT, from the internal region of the protein sequence according to NP_002962.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Thymus shows select nuclear staining. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx. 90 kDa band observed in nuclear lysates of cell lines A431 and Jurkat and in lysates of cell lines U251 and Daudi (calculated MW of 89.0 kDa according to NP_001182399.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur SATB1

Antigène: SATB1 (SATB Homeobox 1 (SATB1))

Autre désignation: SATB1

Sujet: SATB1, SATB homeobox 1, DNA-binding protein SATB1, special AT-rich sequence binding protein 1, special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA's)

ID gène: 6304, 20230, 316164

NCBI Accession: NP_002962

Pathways: Caspase Cascade in Apoptosis, Activated T Cell Proliferation