LIG1 anticorps (Internal Region)

N° du produit ABIN571018

Cet anticorps Chèvre Polyclonal détecte spécifiquement LIG1 dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour LIG1 anticorps (Internal Region) (ABIN571018)

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp LIG1 est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-LIG1 anticorps

Épitope: Internal Region

Fonction: LIG1 / DNA ligase I

Séquence: RVREDKQPEQ ATTS

Réactivité croisée: Humain, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-RVREDKQPEQATTS, from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 125 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102 kDa according to NP_000225.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur LIG1

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Autre désignation: LIG1

Sujet: LIG1, ligase I, DNA, ATP-dependent, MGC117397, MGC130025, DNA ligase I

ID gène: 3978, 81513

NCBI Accession: NP_000225

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA