Calnexin anticorps (Internal Region)

N° du produit ABIN571025

L’anticorps Chèvre Polyclonal anti-Calnexin a été validé pour WB, IHC, IF et ELISA. Il convient pour détecter Calnexin dans des échantillons de Humain et Rat.

Aperçu rapide pour Calnexin anticorps (Internal Region) (ABIN571025)

Antigène: Calnexin (CANX)

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Calnexin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Classe de qualité: Verified

Détail du produit anti-Calnexin anticorps

Épitope: Internal Region

Fonction: Calnexin

Séquence: SKTPELNLDQ FHDKT

Specificité: Reported variants represent identical protein (NP_001019820.1, NP_001737.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-SKTPELNLDQFHDKT, from the internal region (near N Terminus) of the protein sequence according to NP_001737.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Cortex. Recommended concentration: 6 μg/mL.

Western Blot: Approx 90-100 kDa band observed in Human Cerebellum and Rat Brain lysates and in lysates of cell lines LNCaP and U251 (calculated MW of 67.6 kDa according to Human NP_001737.1 and 67.3 kDa according to Rat NP_742005.1). This molecular weight i

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of U251 cells and in the endoplasmic reticulum and plasma membranes of LNCaP cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Calnexin

Antigène: Calnexin (CANX)

Autre désignation: CANX

Sujet: CANX, calnexin, CNX, FLJ26570, IP90, P90, major histocompatibility complex class I antigen-binding protein p88

ID gène: 821, 12330, 29144

NCBI Accession: NP_001737

Pathways: Signalisation MAPK, Thyroid Hormone Synthesis