PRDM1 anticorps (Internal Region)

N° du produit ABIN571072

Cet anticorps Chèvre Polyclonal détecte spécifiquement PRDM1 dans WB, ELISA, IHC, FACS et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour PRDM1 anticorps (Internal Region) (ABIN571072)

Antigène: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PRDM1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-PRDM1 anticorps

Épitope: Internal Region

Fonction: PRDM1 / MEL1

Séquence: DISDNADRLE DVED

Specificité: This antibody is expected to recognize both reported isoforms (NP_001189.2, NP_878911.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DISDNADRLEDVED, from the internal region of the protein sequence according to NP_001189.2, NP_878911.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 6 μg/mL.

Western Blot: Approx 90 kDa band observed in lysates of cell lines A431 and Nuclear K562, and approx. 100 kDa in lysates of cell line K562. An additional faint band of approx 70 kDa was also seen in some A431 and K562 lysates (calculated MW of 91.8 kDa accor

Peptide ELISA: antibody detection limit dilution 1:1000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur PRDM1

Antigène: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Autre désignation: PRDM1

Sujet: PRDM1, PR domain containing 1, with ZNF domain, BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1, B-lymphocyte-induced maturation protein 1, OTTHUMP00000016918, PR-domain zinc finger protein 1, PRDI-binding factor-1, beta-interferon gene posit

ID gène: 639, 12142, 309871

NCBI Accession: NP_001189, NP_878911

Pathways: Regulation of Muscle Cell Differentiation