PLA2G4A anticorps (Internal Region)

N° du produit ABIN571180

Cet anticorps Chèvre Polyclonal détecte spécifiquement PLA2G4A dans WB, IHC, ELISA, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour PLA2G4A anticorps (Internal Region) (ABIN571180)

Antigène: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PLA2G4A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-PLA2G4A anticorps

Épitope: Internal Region

Fonction: PLA2G4A

Séquence: QEKTFRQQRK EHIR

Réactivité croisée: Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QEKTFRQQRKEHIR, from the internal region of the protein sequence according to NP_077734.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 80-85 kDa band observed in lysates of cell line A549 (calculated MW of 85.2 kDa according to NP_077734.1). Preliminary experiments also showed an 85- 90 kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur PLA2G4A

Antigène: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Autre désignation: PLA2G4A

Sujet: PLA2G4A, phospholipase A2, group IVA (cytosolic, calcium-dependent), MGC126350, PLA2G4, cPLA2-alpha, calcium-dependent phospholipid-binding protein, cytosolic phospholipase A2, group IVA, lysophospholipase, phosphatidylcholine 2-acylhydrolase

ID gène: 5321

NCBI Accession: NP_077734

Pathways: Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling