LSD1 anticorps

N° du produit ABIN577699

Cet anticorps Lapin Polyclonal détecte spécifiquement LSD1 dans WB et IP. Il présente une réactivité envers Humain et Mammifères.

Aperçu rapide pour LSD1 anticorps (ABIN577699)

Antigène: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Reactivité: Humain, Mammifères

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LSD1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-LSD1 anticorps

Marque: AbX™

Attributs du produit: Reacts with mammalian Lysine Specific Demethylase 1

Purification: Purified

Immunogène: Full length recombinant Lysine Specific Demethylase 1

Information d'application

Indications d'application: Western blotting and Immunoprecipitation
Western blotting, 1:50-1:200 AbX™ Anti-LSD1 Rabbit pAb
Detection of LSD1 in nuclear extract of 20 μg of HeLa cells probed with A003-100UL at a 1:200 dilution. MW of LSD1 is 110 kDa.

Commentaires:

Reacts with mammalian Lysine Specific Demethylase 1 Supplied as a PBS solution Applications include Western blotting and Immunoprecipitation

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate Buffered Saline at pH 7.2 containing 0.1 % Tween 20 and 0.09 % Kathon preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C

Stockage commentaire: Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Détails sur LSD1

Antigène: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Autre désignation: Lysine Specific Demethylase 1 (LSD1)

Sujet: There are two known classes of histone demethylases (HDM), differing in their reaction chemistry, coenzyme use and reaction products. Flavin-dependent HDMs act only on mono- and dimethylated lysines and produce hydrogen peroxide, whereas the Jumonji-containing HDMs are iron-dependent enzymes that can act on mono-, di- and trimethylated lysine side chains, and also on methylated Arginine residues. Both classes also produce formaldehyde as a product of the demethylation reaction. LSD1 (Lysine-specific demethylase 1) is a flavin- dependent amine oxidase and was identified as a subunit of different complexes and has been shown to be involved in transcriptional repression of genes through the demethylation of monomethyl Lys4 and dimethyl Lys4 Histone H3. LSD1 is also involved in transcriptional activation through the demethylation of monomethyl Lys9 and dimethyl Lys9 Histone H3. It is also able to demethylate both mono- and dimethyl Lys370 in the regulatory domain of the tumor suppressor p53.

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, L'effet Warburg