ADAM10 anticorps (APC)

N° du produit ABIN602624

L’anticorps Souris Monoclonal anti-ADAM10 a été validé pour FACS. Il convient pour détecter ADAM10 dans des échantillons de Humain.

Aperçu rapide pour ADAM10 anticorps (APC) (ABIN602624)

Antigène: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ADAM10 est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Clone: 163003

Détail du produit anti-ADAM10 anticorps (APC)

Specificité: Recognizes human ADAM10 (APC).

Purification: Purified

Immunogène: Mouse myeloma cell line NS0-derived recombinant human ADAM10 (aa Thr214 - Glu672, UniProt Accession #O14672).

Isotype: IgG2b

Information d'application

Indications d'application: Approved: Flo

Usage: Suitable for use in Flow Cytometry Flow Cytometry: Intracellular staining antibodies are designed for multiparameter flow cytometric analysis of cells. To stain for surface proteins (e. g. CD3, CD4, CD8) in addition to the intracellular protein, we recommend that the investigator determine whether the fixation and permeabilization steps adversely affect the surface protein. If so, surface staining of cells prior to fixation and permeabilization is recommended. For intracellular staining, cells must first be fixed and permeabilized. Use of 4 % paraformaldehyde in PBS as a fixative is recommended. Other formulations or tissue fixatives may affect the staining properties of the monoclonal antibody. For permeabilization, 0.1 % saponin in a balanced salt solution is effective in facilitating antibody entry into cells. Due to the reversible nature of cell membrane permeabilization, saponin must be included in all buffers used (i. e. both the staining and washing steps). 10 μL of undiluted product can be used to stain 1 x 10e6 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized from 25 μg antibody, 1 mL PBS, 0.1 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing

Stock: 4 °C,-20 °C

Stockage commentaire: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C.

Détails sur ADAM10

Antigène: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Autre désignation: ADAM10

Sujet: Name/Gene ID: ADAM10
Subfamily: Metallopeptidase M12B
Family: Protease

Synonyms: ADAM10, AD10, ADAM 10, Kuzbanian, HsT18717, CD156c, CD156c antigen, CDw156, Kuz, Kuzbanian protein homolog, MADM

ID gène: 102

UniProt: O14672

Pathways: Signalisation Notch, EGFR Signaling Pathway