ADAM10 anticorps (AA 214-672) (Carboxyfluorescein (CFS))

N° du produit ABIN602625

Cet anticorps anti-ADAM10 est un anticorps Souris Monoclonal détectant ADAM10 dans FACS. Adapté pour Humain.

Aperçu rapide pour ADAM10 anticorps (AA 214-672) (Carboxyfluorescein (CFS)) (ABIN602625)

Antigène: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ADAM10 est conjugé à/à la Carboxyfluorescein (CFS)

Application: Flow Cytometry (FACS)

Détail du produit anti-ADAM10 anticorps (Carboxyfluorescein (CFS))

Épitope: AA 214-672

Specificité: Recognizes human ADAM10.

Purification: Purified

Immunogène: Recombinant human ADAM10, amino acids 214-672, UniProt accession #O14672

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Information d'application

Indications d'application: Approved: Flo

Usage: Suitable for use in Flow cytometry: Intracellular staining antibodies are designed for multiparameter flow cytometric analysis of cells. To stain for surface proteins (e. g. CD3, CD4, CD8) in addition to the intracellular protein, we recommend that the investigator determine whether the fixation and permeabilization steps adversely affect the surface protein. If so, surface staining of cells prior to fixation and permeabilization is recommended. For intracellular staining, cells must first be fixed and permeabilized. Use of 4 % paraformaldehyde in PBS as a fixative is recommended. Other formulations or tissue fixatives may affect the staining properties of the monoclonal antibody. For permeabilization, 0.1 % saponin in a balanced salt solution is effective in facilitating antibody entry into cells. Due to the reversible nature of cell membrane permeabilization, saponin must be included in all buffers used (i. e. both the staining and washing steps). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing

Stock: 4 °C,-20 °C

Stockage commentaire: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.

Détails sur ADAM10

Antigène: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Autre désignation: ADAM10

Sujet: Name/Gene ID: ADAM10
Subfamily: Metallopeptidase M12B
Family: Protease

Synonyms: ADAM10, AD10, ADAM 10, Kuzbanian, HsT18717, CD156c, CD156c antigen, CDw156, Kuz, Kuzbanian protein homolog, MADM

ID gène: 102

UniProt: O14672

Pathways: Signalisation Notch, EGFR Signaling Pathway