IDUA anticorps (AA 26-653)

N° du produit ABIN603316

Cet anticorps Souris Monoclonal détecte spécifiquement IDUA dans ELISA et WB. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour IDUA anticorps (AA 26-653) (ABIN603316)

Antigène: IDUA (Iduronidase, alpha-L- (IDUA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IDUA est non-conjugé

Application: ELISA, Western Blotting (WB)

Clone: 452619

Détail du produit anti-IDUA anticorps

Épitope: AA 26-653

Specificité: Recognizes human Alpha-L-Iduronidase. Species sequence Homology: Mouse (79 % ), chicken (61 %).

Purification: Protein G purified

Immunogène: NS0-derived, recombinant human IDUA aa26-653.

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Direct ELISA: 0.5-1 μg/mL. The detection limit is ~1 ng/well. Antibody was tested using full-length, recombinant protein. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit is ~5 ng/lane under non-reducing and reducing conditions. Antibody tested in WB using the full-length recombinant protein. Chemiluminescent detection will increase sensitivity by 5-50 fold.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Conseil sur la manipulation: Aliquot to Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Références pour anticorps anti-IDUA (ABIN603316)

Gutierrez-Valdes, Häkkinen, Lemasson, de Groot, Ele-Ekouna, Guillet, Cardon, Ritala: "Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process." dans: Biotechnology and bioengineering, (2022) (PubMed).

Cardon, Pallisse, Bardor, Caron, Vanier, Ele Ekouna, Lerouge, Boitel-Conti, Guillet: "Brassica rapa hairy root based expression system leads to the production of highly homogenous and reproducible profiles of recombinant human alpha-L-iduronidase." dans: Plant biotechnology journal, Vol. 17, Issue 2, pp. 505-516, (2019) (PubMed).

Détails sur IDUA

Antigène: IDUA (Iduronidase, alpha-L- (IDUA))

Autre désignation: IDUA / MPS1

Sujet: Name/Gene ID: IDUA

Synonyms: IDUA, Alpha-L-iduronidase, MPS1, Iduronidase, alpha-L-, IDA

ID gène: 3425

Pathways: Glycosaminoglycan Metabolic Process