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HLA-DP beta anticorps (CF®594)

L’anticorps Souris Monoclonal anti-HLA-DP beta a été validé pour FACS, IF et IHC (f). Il convient pour détecter HLA-DP beta dans des échantillons de Humain.
N° du produit ABIN6153325

Aperçu rapide pour HLA-DP beta anticorps (CF®594) (ABIN6153325)

Antigène

HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))

Reactivité

Humain

Hôte

  • 17
Souris

Clonalité

  • 17
Monoclonal

Conjugué

  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Cet anticorp HLA-DP beta est conjugé à/à la CF®594

Application

Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone

BRA-FB6
  • Fonction

    Mouse Monoclonal anti-MHC-II (HLA-DP beta chain) (BRA-FB6), CF594 Conjugate

    Attributs du produit

    This antibody recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked i± (33-35 kDa) and i² (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogène

    Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

    Isotype

    IgG2b kappa
  • Indications d'application

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    Commentaires

    Raji cells. Tonsil or lymph node

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    100 μg/mL

    Buffer

    PBS/0.1 % BSA/0.05 % azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Protect from light
  • Antigène

    HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))

    Autre désignation

    MHC-II (HLA-DP) (beta chain)

    Poids moléculaire

    33-35 kDa

    ID gène

    3115, 347270

    UniProt

    P04440
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