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HLA-DP beta anticorps

L’anticorps Souris Monoclonal anti-HLA-DP beta a été validé pour FACS, IF et IHC (f). Il convient pour détecter HLA-DP beta dans des échantillons de Humain.
N° du produit ABIN6153376

Aperçu rapide pour HLA-DP beta anticorps (ABIN6153376)

Antigène

HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))

Reactivité

Humain

Hôte

  • 17
Souris

Clonalité

  • 17
Monoclonal

Conjugué

  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Cet anticorp HLA-DP beta est non-conjugé

Application

Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone

Bra-14
  • Fonction

    Mouse Monoclonal anti-MHC-II (HLA-DP beta chain) (Bra-14), Purified, BSA-free

    Attributs du produit

    This antibody recognizes a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Purification

    Purified

    Immunogène

    Human REH cells

    Isotype

    IgG3 kappa
  • Indications d'application

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    Commentaires

    Raji cells. Tonsil or lymph node

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS (no BSA, no azide)

    Agent conservateur

    Azide free
  • Antigène

    HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))

    Autre désignation

    MHC-II (HLA-DP) (beta chain)

    Poids moléculaire

    36 kDa (? chain) and 27 kDa (? chain)

    ID gène

    3115, 3122
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