ENO2/NSE anticorps (AA 416-433) (Biotin)

N° du produit ABIN6153634

Détail du produit anti-ENO2/NSE anticorps (Biotin)

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Épitope: AA 416-433

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ENO2/NSE est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: Mouse Monoclonal anti-NSE-gamma (ENO2/1375), Biotin Conjugate

Attributs du produit: This antibody recognizes a protein of about 50 kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: A synthetic peptide corresponding to aa416-433 of human NSE gamma (exact sequence is proprietary)

Clone: ENO2-1375

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistology (formalin) 0.1-0.2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur ENO2/NSE

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Autre désignation: NSE-gamma (ENO2 Produits)

Synonymes: anticorps ENO2, anticorps DKFZp459B1817, anticorps NSE, anticorps AI837106, anticorps D6Ertd375e, anticorps Eno-2, anticorps RNEN3, anticorps eno3, anticorps wu:fc09h05, anticorps zgc:92418, anticorps enolase 2, anticorps enolase 2 (gamma, neuronal), anticorps enolase 2, gamma neuronal, anticorps enolase 2, gamma, neuronal, anticorps ENO2, anticorps Eno2, anticorps eno2

Sujet: 2-phospho-D-glycerate hydrolyase, ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE

Poids moléculaire: ~50 kDa

ID gène: 2026, 511915

UniProt: P09104